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KMID : 1101720140180010001
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Physical Activity and Nutrition
2014 Volume.18 No. 1 p.1 ~ p.7
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The effect of SIRT1 protein knock down on PGC-1 α acetylation during skeletal muscle contraction
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Park Dae-Ryoung
Kim Jeong-Seok
Kim Chang-Keun
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Abstract
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[Purpose] The purpose of this study was to investigate the effect of Sirtuin 1 (SIRT1) and General control nonderepressible 5 (GCN5) knock down on peroxisome proliferator- activated receptor gamma coactivator 1-alpha (PGC-1α) deacetylation during electrical stimulated skeletal muscle contraction.
[Methods] Skeletal muscle primary cell were isolated from C57BL/6 mice gastrocnemius and transfected lentiviral SIRT1 and GCN5 shRNA. Knock downed muscle cell were stimulated by electrical stimulation (1Hz, 3min) and collected for PGC-1α deceatylation assays. Immunoprecipitation performed for PGC-1α deacetylation, acetyl-lysine level was measured.
[Results] Our resulted showed SIRT1 knock down not influenced to PGC-1α deacetylation during electrical stimulation induced muscle contraction while GCN5 knock down decreased PGC-1α deacetylation significantly (p <0.05).
[Conclusion] This study can be concluded that GCN5 is a critical factor for muscle contraction induced PGC-1α deacetylation.
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KEYWORD
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SIRT1, p-AMPK, p-ACC, GCN5, Deacetylation of PGC-1
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